报告题目：Protein Nano- and Micro-tubes as Smart Biomaterials

报告人：小松晃之  Teruyuki Komatsu 教授（日本中央大学）

报告时间：2015-5-15  15:30

报告地点：实验一楼，第一会议室

联系人：屈雪

欢迎您参加！

报告人介绍：
    小松晃之 教授担任日本中央大学理工学院应用化学系教授。主要从事功能性生物大分子研究。
研究兴趣：
    1.Synthesis of protein nano- and micro-architectures as smart biomaterials
    2.Synthesis and medical applications of artificial O2-carriers as a red blood cell substitute
    3.Synthesis of metalloporphyrin architectures by coordination chemistry

报告摘要：
    Synthesis of cylindrical hollow structures in nanometer- or micrometer-scale using biomolecules, such as amphiphilic lipids, peptides, DNAs, and proteins, has attracted considerable attentions because of their potential applications. I will present highlights of our latest results of studies directed at developing protein-based nanotubes (NTs) and microtubes (MTs) as smart biomaterials.1-6 A variety of guest molecules can be encapsulated into their one-dimensional (1D) pore space selectively via biospecific interaction. These biocylinders were prepared using an alternating layer-by-layer assembly of protein and oppositely charged poly(amino acid) into a nano- or micro-porous polycarbonate membrane (pore diameter: 0.4~1.0 μm), with subsequent dissolution of the template.2 The tubular wall typically comprises six layers of poly-L-arginine (PLA) and human serum albumin (HSA) [(PLA/HSA)3]. The obtained (PLA/HSA)3 NTs (outer diameter: 400 nm) can be dispersed in aqueous medium and are hydrated significantly. The hybrid NTs bearing a single avidin layer as an internal wall captured biotin-labeled nanoparticles into the central channel when their particle size is sufficiently small to enter the pores.2 The NTs with an antibody surface interior entrapped human hepatitis B virus with size selectivity.3 It is noteworthy that the infectious Dane particles were completely encapsulated into the hollows. More recently, we synthesized protein MTs (outer diameter: 1 μm) having bacteria trapping ability.4 Confocal laser scanning microscopy measurements clearly showed that Escherichia coli was entrapped into the hollow of the (PLA/HSA)9 MT.